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labs and their techniques? 8 years 4 months ago #13147
Hi there, I am in the middle of Mexico here and I am trying to learn what kind of lab techniques I should be looking for to get my VL results from here on out. I finished treatment almost 2 weeks ago. A lab guy here (they send out the blood so he doesn't know much about it) thought there were different techniques/machines used to get the results and that possibly some are more accurate than others. I would like to be able to call around to different labs in the country and make sure they are using the best methods possible to read my VL or lack thereof. Anybody have a clue what that might be? thanks, Ken HCV 35 yrs G1a F3 Tx naive started Lesovir-C 15/12/2015 pre tx: VL 5,250,000 ALT 374 AST 208 FIBROSCORE 10.4 4 weeks tx ALT 29/ AST 33. VL < 12 UI/mL 8 weeks tx ALT 29/ AST 34. VL UND 4 weeks after tx UND. SVR4. ALT 24/AST 18 | |
labs and their techniques? 8 years 4 months ago #13148
| Hi ken, Dr Freeman gave me this info : Abbot and Roche make the PCR test. The usual one here is the Roche TaqMan COBAS molecular.roche.com/assays/Pages/COBASTaqManHCVTestv20HPS.aspx The tests I order are either HCV RNA PCR quantitative HCV RNA PCR qualitative GT1a Dec14 F2/8.7 VL 900000-2.5M Jan16 Hepcivir-L MonkMed/Redemption Baseline: VL 913575 Alt 76 Platelets low Wk2 VL1157 Alt 23 DET Wk 8 VL 32 Alt19 'In the slow lane' June16 Fibro 5.7 F0/1 LIF 1.5 Wk 11 VL<12 Alt 13 Det/Unq Extending tx 12 wks Mylan Sofo/Dac MonkMed Wk 14 VL <12 Det/Unq Wk 16 VL UNDETECTED Wk 22 + 4 Wks Sunprevir FixHepC Wk 24 UNDETECTED Alt 13 Wk 12 post tx SVR12 Wk 26 SVR24 Thank-you Tim, Dr Debasis @ MonkMed & Dr Freeman @ Fix HepC |
labs and their techniques? 8 years 4 months ago #13149
Where I live its state provided and they use the TaqMan COBAS which <25 as LLOQ The link is provided above by LG Features CE-IVD: Sensitivity (LOD; genotype 1) – plasma: 9.3 HCV RNA IU/mL, serum: 8.8 HCV RNA IU/mL; linear range – 25 to 3.91×10^8 IU/mL FDA-IVD: Sensitivity (LoD; across all genotypes) – 20 IU/mL; Linear range –Quantitation of HCV RNA from 25 to 3×10^8 IU/mL COBAS® TaqMan® 48 Analyzer for automated real-time PCR amplification and detection Treatment naive F 3/4 Genotype 1 a & b V/L 17 MILLION Started Harvoni 11th Dec 2015 for 12 weeks 4 weeks VL UND 6 WEEKS ALT 32, AST 34 EOT 03/03 2016 ! UND ALT 34, AST 26 04.04.2016 SVR 4 26.05.2016 SVR 12 16.08.2016 SVR 24 The following user(s) said Thank You: kenbasman | |
labs and their techniques? 8 years 4 months ago #13164
| The lab I use, Quest Diagnostics, only has RNA & PCR RNA Quantitative. These were my results. HCV RNA IU/mL <15 Not Detected HCV RNA,QN,PCR <1.18 Not Detected Edit: Apparently the first test, RNA IU/mL is the Qualitative result and shows no active infection. The second test is quantitative and confirms it! Gt 1a, F0, VL 6.5 million, AST 59, ALT 62 Started Twinvir 1/15/16 6 wk. labs VL UND, AST 27, ALT 20 EOT labs VL UND, AST 23, ALT 19 SVR 16, VL UND, AST 28, ALT 17 SVR 24 , VL UND, 10/8/16 SVR 125, VL UND, 9/22/18 SVR 230, VL UND, 10/3/20 |
labs and their techniques? 8 years 4 months ago #13172
Thanks everybody. I thnk i begin to know whqt to look for, Ken HCV 35 yrs G1a F3 Tx naive started Lesovir-C 15/12/2015 pre tx: VL 5,250,000 ALT 374 AST 208 FIBROSCORE 10.4 4 weeks tx ALT 29/ AST 33. VL < 12 UI/mL 8 weeks tx ALT 29/ AST 34. VL UND 4 weeks after tx UND. SVR4. ALT 24/AST 18 | |
labs and their techniques? 8 years 4 months ago #13179
As in my case <25 and a note UNDETECTED at the LLOQ. Abbot test is <15 LLOQ Roche Cobas Taqman <25 LLOQ AND if its detected it will mention as detected but sometimes the guy who writes the report forgets to write UNDETECTED <15, <25 <...... SO DONT PANIC Treatment naive F 3/4 Genotype 1 a & b V/L 17 MILLION Started Harvoni 11th Dec 2015 for 12 weeks 4 weeks VL UND 6 WEEKS ALT 32, AST 34 EOT 03/03 2016 ! UND ALT 34, AST 26 04.04.2016 SVR 4 26.05.2016 SVR 12 16.08.2016 SVR 24 The following user(s) said Thank You: Fara | |
labs and their techniques? 8 years 4 months ago #13180
Unless you are one of the rare few who had normal/near normal LFTs pre treatment in all the relapses I have seen I've known it's coming a week in advance because the LFTs are off again. A few patients scared me with elevated enzymes but came back undetected - this traced back to celebrating with alcohol. Any of the viral load tests will pick up a relapse. YMMV The following user(s) said Thank You: kenbasman, LondonGirl, mgalbrai, Thurl, Gaj, Lynne-Francis-facebook, isaing4, Fara | |
labs and their techniques? 7 years 9 months ago #22102
Dear Dr.Freeman As you mentioned that very few have normal or near normal liver function tests and as I am one of those I wondered whether you have observed any particular traits of disease which presents with LFTs being normal. My ALT is 45 , viral load about 400 000 , no symptoms but fibroscan is 12. Is there anything that could be read into this unusual combination or it is of no relevance? Thank you. If anybody else has a clue the replies are welcome. Diagnosed September 2016. 1b ALAT in 40s. VL 460 000 Fibroscan 12.5 Start of treatment 18/10/16 Wk 2 VL 145 Wk 4 VL detected unquantifiable Wk 8 VL detected unquantifiable Wk 12 undetected week 30 after eot - undetected | |
labs and their techniques? 7 years 9 months ago #22121
Hep C can be remarkably unsymptomatic until it isn't. Many people can wait for treatment. @12kPa you are almost cirrhotic. For you waiting may be a mistake. YMMV | |
labs and their techniques? 7 years 9 months ago #22129
O yes I can not wait to start trratement , I hope medicines would be shipped tomorrow and my immense gratitude to you for giving me this opportunity. Just been pondering trying to make sense of me and my hepatitis Diagnosed September 2016. 1b ALAT in 40s. VL 460 000 Fibroscan 12.5 Start of treatment 18/10/16 Wk 2 VL 145 Wk 4 VL detected unquantifiable Wk 8 VL detected unquantifiable Wk 12 undetected week 30 after eot - undetected | |
labs and their techniques? 7 years 9 months ago #22141
Countless you are in the right place at the right time. Keep us posted! Your almost there, Congrats! Contracted HCV 1980's Geno Type 1a F3 ( doc says once treated I'll be F2 maybe F1) Meds shipped 6/17/2016 arrived early 7/2016 Viral count - 3,471,080 4 week quantitative bloods: August 17, 2016. I have been diagnosed as <15 (told undetected) 8 week quantitative bloods: September 14th. I have been diagnosed as <15 (told undetected) 11 week PCR RNA Qualitative bloods: September 26th 2016 - Undetected December 19th 2016: Cured! Viral count: zero!!! 2018 viral count: still zero! Cured! The following user(s) said Thank You: Stephen-Powell-facebook, fitz | |
labs and their techniques? 7 years 9 months ago #22382
I did not exactly realise that 2 years ago neither these medications nor this site existed , what you saying makes sense now.. Diagnosed September 2016. 1b ALAT in 40s. VL 460 000 Fibroscan 12.5 Start of treatment 18/10/16 Wk 2 VL 145 Wk 4 VL detected unquantifiable Wk 8 VL detected unquantifiable Wk 12 undetected week 30 after eot - undetected | |
labs and their techniques? 7 years 9 months ago #22383
Yes Countless, Welcome to the tip of the spear in the battle to wipe out HCV. As Sven says, the time is right. Good luck, m This email address is being protected from spambots. You need JavaScript enabled to view it. forums.delphiforums.com/generichcvtx G 1a F-1 Started tx 10/23/15 (Meso sof & led) ALT 48 AST 28 v/l 1.6 mil 11/17/15 4 wk lab ALT 17 AST 16 <15 11/18/15 Started Harvoni 12/16/15 8 wk lab ALT: 15 AST: 13 V/l UND 1/14/16 Fin. Tx 7/07/16 UND SVR 24 | |
labs and their techniques? 7 years 8 months ago #22486
Thank you. On the topic of lab results - does anybody know how to convert viral load counts in units into ones in fmols and viceversa ? Diagnosed September 2016. 1b ALAT in 40s. VL 460 000 Fibroscan 12.5 Start of treatment 18/10/16 Wk 2 VL 145 Wk 4 VL detected unquantifiable Wk 8 VL detected unquantifiable Wk 12 undetected week 30 after eot - undetected | |
labs and their techniques? 7 years 8 months ago #22490
. I haven't seen VL expressed as fmols, do you mean Log10 scale? In which case it is a logarithmic progression which I don't have the skills to explain how to calculate but can supply the below approximations. (Sorry about the wonky columns, too much work to tabulate on an iPad Log10 iu/mL 7.0 10,000,000 6.9 7,943,282 6.8 6,309,573 6.7 5,011,872 6.6 3,981,072 6.5 3,162,278 6.4 2,511,886 6.3 1,995,262 6.2 1,584,893 6.1 1,258,925 6.0 1,000,000 5.9 794,328 5.8 630,957 5.7 501,187 5.6 398,107 5.5 316,228 5.4 251,189 5.3 199,526 5.2 158,489 5.1 125,893 5.0 100,000 4.9 79,433 4.8 63,096 4.7 50,119 4.6 39,811 4.5 31,623 4.4 25,119 4.3 19,953 4.2 15,849 4.1 12,589 4.0 10,000 3.9 7,943 3.8 6,310 3.7 5,012 3.6 3,981 3.5 3,162 3.4 2,512 3.3 1,995 3.2 1,585 3.1 1,259 3.0. 1,000 2.9 794 2.8 631 2.7 501 2.6 398 2.5 316 2.4 251 2.3 200 2.2 158 2.1 126 2.0 100 1.9 79 1.8 63 1.7 50 1.6 40 1.5 32 1.4 25 1.3 20 1.2 16 1.1 13 1.0 10 0.9 8 0.8 6 0.7 5 0.6 4 0.5 3 0.4 3 0.3 2 0.2 2 0.1 1 G3a since '78 - Dx '12 - F4 (2xHCC) 24wk Tx - PEG/Riba/Dac 2013 relapsed 24wk Tx - Generic Sof/Dac/Riba 2015/16 relapsed 16wk Tx - 12/01/17 -> 03/05/17 NS3/NS5a + Generic Sof SVR7 - 22/06/17 UND SRV12 - 27/07/17 UND SVR24 - 26/10/17 UND | |
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