Please see the attached NMR results for Mesochem Daclatasvir, which I have got done from my local university's chemistry lab. There were two tests done, one is against Hydrogen and other one against Carbon. As per the NMR technician it confirms the molecular structure of Daclastsvir but there is some minor impurity but he failed to quantify it as he was not expert on the subject. I am posting the results here to seek more expert comments.
NMR Analyser used was Brukker 300 MHZ (don't know if there are any further classification)
Mesochem Daclatasvir NMR results - pls interpret
8 years 2 months ago #605
So if you look at your spectrum you see 12.66 @ around 0.85 ppm - this represents 2 peaks (0.79 & 0.88) merged into one due to instrument power limitations. You can continue on for other peaks, but your spectrum is not great one for teaching NMR basics.
I have attached a pdf showing the impact of concentration on the Daclatasvir spectrum. This was a dilution of exactly the same sample so the only thing different was more solvent. Notice that despite the fact it remained the same API there have been changes to the spectrum.